Transcriptome
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> Services
Different Services are offered: nucleic acid and protein extraction, nucleic acid quantification, total RNA quality control, cDNA synthesis with quality control (protocols adapted to RNA quantities), design and validation of PCR primers (More than 5000 pairs of primers for different species), quantitative PCR with the chemistries SybrGreen or Taqman probes, digital PCR with the chemistries Evagreen or Taqman probes (Absolute quantity), selection of the appropriate controls for the qPCR and dPCR, PCR on unique cell (from patch clamp, cell sorting, laser microdissection...)…
Different protocols (from RNA to the PCR reaction) are designed for small (microdissected brain structures) or large materials.
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> Financial support of LabEx BRAIN
- 2020 : 45 719 €
- 2019 : 45 719 €
- 2018 : 45 719 €
- 2017 : 45 719 €
- 2016 : 55 083 €
- 2015 : 57 283 €
- 2014 : 57 283 €
- 2013 : 57 283 €
- 2012 : 57 283 €
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> Reduction in utilization costs of Bordeaux Neurocampus users
Thanks to the LabEx grant, users belonging to Bordeaux Neurocampus benefit from a discount ranging from 20 to 40% depending on the service offered.
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> Contact
Head of the platform : Thierry LESTE-LASSERRE / thierry.leste-lasserre@inserm.fr