Université de Bordeaux
LabEx BRAINBordeaux Region Aquitaine Initiative for Neurosciences
Cluster of excellence

Biochemistry

> Platform website

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> Services

Provision of engineering equipment and services:

  • Protein production and purification (affinity and steric exclusion columns)

  • Analysis of the size and molecular weight of proteins purified by DLS and SLS

  • Analysis of biomolecular interactions by isothermal calorimetric titration (determination of affinity constant, stoichiometry and thermodynamic parameters)

  • Cell crushing (bacteria, eukaryotic cells, tissues)

  • Centrifugation and Ultracentrifugation

  • Determination of protein concentration by infrared

  • Electrophoresis and membrane transfer

  • Digital analysis of gels and blots (colorimetry, chemiluminescence)

  • Water purification system

  • Plate readers (UV absorption, fluorescence intensity, fluorescence lifetime, fluorescence polarization, luminescence. Applications include the quantification of nucleic acids and proteins, ELISA, FRET, enzyme activity tests, and enzyme kinetics)

  • MAGPIX (Biorad) plate reader for ELISA tests that works on magnetic beads and can identify and quantify 50 target molecules per well on 96-well plate, or 4800 tests in total. Many kits are available but you can design your own

  • Ingenuity Software (QIAGEN), this is a manually annotated bank that allows you to quickly analyze and interpret transcriptomic and proteomic data. It is also a very effective bibliographic tool

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> Financial support of LabEx BRAIN

  • 2019 : 64 534 €
  • 2018 : 109 533 €
  • 2017 : 64 533 €
  • 2016 : 77 750 €
  • 2015 : 70 000 €
  • 2014 : 105 000 €
  • 2013 : 105 000 €
  • 2012 : 105 840 €

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> Reduction in utilization costs for Bordeaux Neurocampus users

About 50%

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> Contacts



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